![]() Batalov et al., PNAS, 2021ĭeForest’s colleagues on this project are lead author Ivan Batalov, a former UW postdoctoral researcher in chemical engineering and bioengineering, and co-author Kelly Stevens, a UW assistant professor of bioengineering and of laboratory medicine and pathology. Black regions were not scanned with the laser, and so the mCherry protein did not adhere to those portions of the hydrogel. The team scanned near-infrared lasers in the shapes of a monster (left) and the Space Needle (right) to create these patterns. Top view of two collagen hydrogels that researchers decorated with immobilized mCherry proteins, which glow red under fluorescent light. “Moreover, it makes use of exceptionally precise photochemistries that can be controlled in 4D while uniquely preserving protein function and bioactivity.” “This approach provides us with the opportunities we’ve been waiting for to exert greater control over cell function and fate in naturally derived biomaterials - not just in three-dimensional space but also over time,” said DeForest. These methods could form the basis of biologically based scaffolds that might one day make functional laboratory-grown tissues a reality, said DeForest, who is also a faculty member with the UW Molecular Engineering and Sciences Institute and the UW Institute for Stem Cell and Regenerative Medicine. The proteins on these biological scaffolds triggered changes to messaging pathways within the cells that affect cell growth, signaling and other behaviors. ![]() Mammalian cells responded as expected to the adhered protein signals within the 3D scaffold, according to senior author Cole DeForest, a UW associate professor of chemical engineering and of bioengineering. 18 in the Proceedings of the National Academy of Sciences, uses a near-infrared laser to trigger chemical adhesion of protein messages to a scaffold made from biological polymers such as collagen, a connective tissue found throughout our bodies. Their approach, published the week of Jan. In a major step toward transforming this hope into reality, researchers at the University of Washington have developed a technique to modify naturally occurring biological polymers with protein-based biochemical messages that affect cell behavior. Black regions were masked from the light, and so the mCherry protein did not adhere to those portions of the hydrogel. The team shined UV light on the hydrogel through a mask cut out in the shape of a former University of Washington logo. Top view of a collagen hydrogel that researchers decorated with immobilized mCherry proteins, which glow red under fluorescent light.
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